Enzymatic and functional analysis of a protein phosphatase, Pph3, from Myxococcus xanthus.
نویسندگان
چکیده
A protein phosphatase, designated Pph3, from Myxococcus xanthus showed the enzymatic characteristics of PP2C-type serine/threonine protein phosphatases, which are metal ion-dependent, okadaic acid-insensitive protein phosphatases. The pph3 mutant under starvation conditions formed immature fruiting bodies and reduced sporulation.
منابع مشابه
The Phosphatomes of the Multicellular Myxobacteria Myxococcus xanthus and Sorangium cellulosum in Comparison with Other Prokaryotic Genomes
BACKGROUND Analysis of the complete genomes from the multicellular myxobacteria Myxococcus xanthus and Sorangium cellulosum identified the highest number of eukaryotic-like protein kinases (ELKs) compared to all other genomes analyzed. High numbers of protein phosphatases (PPs) could therefore be anticipated, as reversible protein phosphorylation is a major regulation mechanism of fundamental b...
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A recombinant Myxococcus xanthus strain was constructed that constitutively produces two proteins from Escherichia coli, the cytoplasmic beta-galactosidase and the periplasmic pH 2.5 acid phosphatase (AppA protein). We have previously shown that during vegetative growth, AppA protein is partly accumulated in the periplasm of M. xanthus and partly released into the medium. We demonstrate here th...
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Functional Myxococcus xanthus A signal-generating and A signal-responding pathways are required for the progression through early multicellular development. To identify genes responsive to these pathways, the expression of eight early developmental genes was analyzed. This examination identified one gene as a target of the A signal-generating pathway and four genes as targets of the A signal-re...
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Myxococcus xanthus involves an extracellular NAD(P)+-containing protein. Genes Dev.9, 2964–2973 8 Kim, S.K. and Kaiser, D. (1990) C-factor: a cellcell signaling protein required for fruiting body morphogenesis of M. xanthus. Cell 61, 19–26 9 Ogawa, M. et al. (1996) FruA, a putative transcription factor essential for the development of Myxococcus xanthus. Mol. Microbiol. 22, 757–767 10 Sogaard-A...
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عنوان ژورنال:
- Journal of bacteriology
دوره 193 10 شماره
صفحات -
تاریخ انتشار 2011